6KW6
Crystal Structure of cytidine deaminase from Streptomyces noursei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-02 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97916 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 67.773, 74.259, 52.785 |
| Unit cell angles | 90.00, 106.57, 90.00 |
Refinement procedure
| Resolution | 29.933 - 1.895 |
| R-factor | 0.1692 |
| Rwork | 0.168 |
| R-free | 0.19220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mpz |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.824 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.933 | 1.930 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmerge | 0.074 | 0.476 |
| Number of reflections | 19489 | 992 |
| <I/σ(I)> | 14.2 | 3.3 |
| Completeness [%] | 98.2 | |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 0.2M Magnesium chloride hexahydrate, 0.1M Tris pH8.5 and 25% w/v Polyethylene glycol 3,350 |
