6KW6
Crystal Structure of cytidine deaminase from Streptomyces noursei
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-02 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97916 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 67.773, 74.259, 52.785 |
Unit cell angles | 90.00, 106.57, 90.00 |
Refinement procedure
Resolution | 29.933 - 1.895 |
R-factor | 0.1692 |
Rwork | 0.168 |
R-free | 0.19220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3mpz |
RMSD bond length | 0.006 |
RMSD bond angle | 0.824 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.933 | 1.930 |
High resolution limit [Å] | 1.890 | 1.890 |
Rmerge | 0.074 | 0.476 |
Number of reflections | 19489 | 992 |
<I/σ(I)> | 14.2 | 3.3 |
Completeness [%] | 98.2 | |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 0.2M Magnesium chloride hexahydrate, 0.1M Tris pH8.5 and 25% w/v Polyethylene glycol 3,350 |