6KVQ

S. aureus FtsZ in complex with BOFP (compound 3)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.900
Spacegroup name	C 1 2 1
Unit cell lengths	72.268, 49.686, 88.592
Unit cell angles	90.00, 111.24, 90.00

Refinement procedure

Resolution	36.091 - 1.600
R-factor	0.1911
Rwork	0.190
R-free	0.21350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3voa
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	36.091	36.091	1.640
High resolution limit [Å]	1.600	7.160	1.600
Rmerge	0.064	0.030	0.881
Rmeas	0.069	0.033	0.950
Total number of observations	265154
Number of reflections	38774	462	2871
<I/σ(I)>	15.09	39.44	1.99
Completeness [%]	99.8	98.3	99.9
Redundancy	6.838	6.639	7.053
CC(1/2)	0.999	0.999	0.715

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	100 mM HEPES, 39% (w/v) PEP629