6KVN

Crystal structure of chloroplast resolvase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 2
Unit cell lengths	62.793, 40.335, 60.504
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.570 - 2.020
R-factor	0.1939
Rwork	0.192
R-free	0.23670
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.774
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14rc3_3206)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.570	2.070
High resolution limit [Å]	2.020	2.020
Rmerge	0.207	0.853
Rmeas	0.226	0.940
Rpim	0.090	0.387
Number of reflections	10538	4218
<I/σ(I)>	6	2.6
Completeness [%]	99.8	98.3
Redundancy	6.3	5.7
CC(1/2)	0.978	0.778

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	Bis-Tris, PEG 3350, Lithium sulphat