6KVN
Crystal structure of chloroplast resolvase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 62.793, 40.335, 60.504 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.570 - 2.020 |
| R-factor | 0.1939 |
| Rwork | 0.192 |
| R-free | 0.23670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.774 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14rc3_3206) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.570 | 2.070 |
| High resolution limit [Å] | 2.020 | 2.020 |
| Rmerge | 0.207 | 0.853 |
| Rmeas | 0.226 | 0.940 |
| Rpim | 0.090 | 0.387 |
| Number of reflections | 10538 | 4218 |
| <I/σ(I)> | 6 | 2.6 |
| Completeness [%] | 99.8 | 98.3 |
| Redundancy | 6.3 | 5.7 |
| CC(1/2) | 0.978 | 0.778 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | Bis-Tris, PEG 3350, Lithium sulphat |
