6KVL
Crystal structure of UDP-RebB-SrUGT76G1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-09-28 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9798 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 97.463, 97.463, 89.899 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.674 - 1.998 |
R-factor | 0.1695 |
Rwork | 0.168 |
R-free | 0.20450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vce |
RMSD bond length | 0.010 |
RMSD bond angle | 1.403 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.674 | 2.070 |
High resolution limit [Å] | 1.998 | 1.998 |
Rmeas | 0.153 | |
Number of reflections | 53258 | 3321 |
<I/σ(I)> | 26.6 | |
Completeness [%] | 100.0 | |
Redundancy | 11 | |
CC(1/2) | 0.994 | 0.941 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 18% (vol/vol) 2-propanol, 0.1M sodium citrate tribasic dihydrate, pH 5.5, 18% (wt/vol) PEG4000 |