6KVE

Crystal structure of a GH28 endo-polygalacturonase from Talaromyces leycettanus JCM 12802

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	44.175, 67.823, 87.991
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.720 - 1.320
R-factor	0.1626
Rwork	0.160
R-free	0.21970
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.005
RMSD bond angle	0.775
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.720	1.370
High resolution limit [Å]	1.320	1.320
Rmerge	0.088	0.750
Rmeas	0.103	0.888
Rpim	0.052	0.462
Number of reflections	60589	5346
<I/σ(I)>	6.63	1.09
Completeness [%]	95.8	84.93
Redundancy	3.7	3.1
CC(1/2)	0.993	0.528

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.8	293	0.1 M citrate buffer (pH 4.8), 28% (w/v) polyethylene glycol monomethyl ether 5000