6KUF
Crystal structure of barley exohydrolaseI W434A mutant in complex with glucose.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-22 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.9537 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 100.693, 100.693, 182.393 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.640 - 1.900 |
R-factor | 0.152 |
Rwork | 0.150 |
R-free | 0.18500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3wli |
RMSD bond length | 0.021 |
RMSD bond angle | 1.993 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 88.150 | 1.950 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.200 | 0.180 |
Number of reflections | 70151 | 70151 |
<I/σ(I)> | 29.8 | |
Completeness [%] | 99.2 | |
Redundancy | 24.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 1.7 M ammonium sulfate, 75 mM HEPES-NaOH buffer, pH 7 |