6KUE

The structure of BioZ from Agrobacterium tumefaciens

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	63.810, 85.780, 110.480
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.443 - 1.992
R-factor	0.1833
Rwork	0.181
R-free	0.22850
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	0.943
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.260	2.063
High resolution limit [Å]	1.990	1.990
Rmerge	0.371	1.114
Number of reflections	41976	4100
<I/σ(I)>	15.8
Completeness [%]	99.5
Redundancy	25.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	COUNTER-DIFFUSION		289.15	0.2M Sodium nitate,20%PEG3,350.