6KRL
Crystal structure of GH30 xylanase B from Talaromyces cellulolyticus expressed by Pichia pastoris
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-01-24 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 63.337, 78.765, 117.839 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.140 - 1.601 |
Rwork | 0.172 |
R-free | 0.19840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6iuj |
RMSD bond length | 0.008 |
RMSD bond angle | 1.483 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP (11.6) |
Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.144 | 1.658 |
High resolution limit [Å] | 1.601 | 1.601 |
Rmerge | 0.093 | 0.801 |
Rpim | 0.039 | 0.332 |
Number of reflections | 78001 | 7458 |
<I/σ(I)> | 11.8 | 2 |
Completeness [%] | 99.6 | 96.61 |
Redundancy | 6.7 | 6.8 |
CC(1/2) | 0.996 | 0.660 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG 3350, HEPES, magnesium chloride |