6KR7
Crystal structure of methylated human leucyl-tRNA synthetase, Leu-AMS-bound form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 273 |
Detector technology | CCD |
Collection date | 2016-10-09 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 1.0000 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 136.818, 136.818, 437.266 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.877 - 4.000 |
R-factor | 0.2366 |
Rwork | 0.232 |
R-free | 0.27760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wz2 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 4.070 |
High resolution limit [Å] | 4.000 | 10.820 | 4.000 |
Rmerge | 0.106 | 0.064 | 0.308 |
Rmeas | 0.112 | 0.067 | 0.348 |
Rpim | 0.034 | 0.019 | 0.153 |
Total number of observations | 145569 | ||
Number of reflections | 20622 | 1237 | 976 |
<I/σ(I)> | 6.7 | ||
Completeness [%] | 95.3 | 97.6 | 93.9 |
Redundancy | 7.1 | 11.5 | 4 |
CC(1/2) | 0.998 | 0.551 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M HEPES (pH 7.1), 0.42 M Ammonium sulfate, 24 % PEG3350 |