6KR0

Crystal structure of HL homo-diabody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-09
Detector	RAYONIX MX225HE
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	87.080, 88.480, 135.440
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.750 - 2.300
R-factor	0.2029
Rwork	0.202
R-free	0.22620
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.750	2.300
High resolution limit [Å]	2.300	2.300
Number of reflections	47159	3394
<I/σ(I)>	27.85
Completeness [%]	100.0
Redundancy	7.43

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.1 M Tirs-HCl pH 9.0, 0.2 M calcium chloride, 3.25 M sodium formate