6KQX

Crystal structure of Yijc from B. subtilis in complex with UDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A1
Synchrotron site	NSRRC
Beamline	BL15A1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-12-11
Detector	RAYONIX MX-300
Wavelength(s)	1.0
Spacegroup name	I 2 2 2
Unit cell lengths	52.152, 116.722, 185.226
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.688 - 2.440
R-factor	0.2316
Rwork	0.230
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3iaa
RMSD bond length	0.004
RMSD bond angle	0.755
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	25.000	25.000	2.530
High resolution limit [Å]	2.440	5.240	2.440
Rmerge	0.051	0.025	0.525
Rmeas	0.058	0.028	0.605
Rpim	0.029	0.014	0.295
Total number of observations	83151
Number of reflections	21112	2285	1819
<I/σ(I)>	15
Completeness [%]	98.4	99.9	87.5
Redundancy	3.9	4	3.7
CC(1/2)		0.999	0.820

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	295	1.2 M tri-Sodium Citrate dehydrate, 0.1 M Bis-Tris Propane