6KQW
Crystal structure of Yijc from B. subtilis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL15A1 |
Synchrotron site | NSRRC |
Beamline | BL15A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-12-11 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 1.0 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 51.819, 116.845, 187.660 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.811 - 2.180 |
R-factor | 0.2246 |
Rwork | 0.223 |
R-free | 0.25000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3iaa |
RMSD bond length | 0.004 |
RMSD bond angle | 0.748 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 25.000 | 25.000 | 2.260 |
High resolution limit [Å] | 2.180 | 4.690 | 2.180 |
Rmerge | 0.052 | 0.021 | 0.730 |
Rmeas | 0.059 | 0.025 | 0.832 |
Rpim | 0.028 | 0.012 | 0.394 |
Total number of observations | 133014 | ||
Number of reflections | 30469 | 3204 | 3012 |
<I/σ(I)> | 14.4 | ||
Completeness [%] | 99.9 | 99.4 | 99.9 |
Redundancy | 4.4 | 4.2 | 4.4 |
CC(1/2) | 0.999 | 0.759 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 1.2 M tri-Sodium Citrate dehydrate, 0.1 M Bis-Tris Propane |