6KQE
Thermus thermophilus initial transcription complex comprising sigma A and 5'-OH RNA of 4 nt
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-07-30 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.075 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 184.567, 103.757, 295.981 |
Unit cell angles | 90.00, 98.70, 90.00 |
Refinement procedure
Resolution | 48.947 - 3.300 |
R-factor | 0.2195 |
Rwork | 0.217 |
R-free | 0.25820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4g7h |
RMSD bond length | 0.008 |
RMSD bond angle | 1.074 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.360 |
High resolution limit [Å] | 3.300 | 8.940 | 3.300 |
Rmerge | 0.132 | 0.036 | 0.955 |
Total number of observations | 282967 | ||
Number of reflections | 83673 | 4355 | 4146 |
<I/σ(I)> | 5 | ||
Completeness [%] | 100.0 | 100 | 99.7 |
Redundancy | 3.4 | 3.3 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.05 M Magnesium chloride, 0.2 M Potassium chloride, 0.1 M Tris-HCl pH8, 10% PEG 4000 |