6KPL
Crystal Structure of endo-beta-N-acetylglucosaminidase from Cordyceps militaris in apo form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-04 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 1.1000 |
Spacegroup name | P 65 |
Unit cell lengths | 105.513, 105.513, 63.432 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.590 - 1.750 |
R-factor | 0.1567 |
Rwork | 0.155 |
R-free | 0.18430 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.756 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.780 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.120 | 0.801 |
Number of reflections | 40703 | 2026 |
<I/σ(I)> | 23.9 | 3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 10.3 | 10.4 |
CC(1/2) | 0.991 | 0.863 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.6 | 277.15 | 0.1 M CHES-NaOH (pH 8.6) and 20 % PEG3350 |