6KPB

The crystal structure of the JACKDAW/IDD10 bound to the homodimeric SCL3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000
Spacegroup name	P 32 2 1
Unit cell lengths	112.037, 112.037, 70.979
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.981 - 2.400
R-factor	0.1921
Rwork	0.191
R-free	0.21400
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.510	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.017	0.631
Rpim	0.011	0.321
Number of reflections	20315	2115
<I/σ(I)>	21.8	1.5
Completeness [%]	99.7	100
Redundancy	4.6	4.8
CC(1/2)	1.000	0.900

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	2-5% 2-propanol, 0.05M tri-sodium citrate, 0.1M HEPES-NaOH (pH 7.0)