6KOP
Mycobacterium tuberculosis initial transcription complex comprising sigma H and 5'-OH RNA of 9 nt
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-29 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.97916 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 127.371, 162.637, 133.421 |
Unit cell angles | 90.00, 117.95, 90.00 |
Refinement procedure
Resolution | 49.252 - 3.303 |
R-factor | 0.2185 |
Rwork | 0.217 |
R-free | 0.25470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5zx3 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.360 |
High resolution limit [Å] | 3.300 | 8.940 | 3.300 |
Rmerge | 0.119 | 0.042 | 1.547 |
Rmeas | 0.134 | 0.048 | 1.748 |
Rpim | 0.061 | 0.023 | 0.797 |
Total number of observations | 299315 | ||
Number of reflections | 70882 | 3553 | 3583 |
<I/σ(I)> | 8.6 | ||
Completeness [%] | 98.6 | 96.7 | 99.2 |
Redundancy | 4.2 | 3.9 | 4.2 |
CC(1/2) | 0.997 | 0.502 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.008 M magnesium acetate, 0.05 M sodium cacodylate pH6.5, 14% PEG 400 |