6KOO
Mycobacterium tuberculosis initial transcription complex comprising sigma H and 5'-OH RNA of 7 nt
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-12 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.97890 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 125.033, 162.314, 128.482 |
Unit cell angles | 90.00, 117.03, 90.00 |
Refinement procedure
Resolution | 31.377 - 2.800 |
R-factor | 0.2073 |
Rwork | 0.206 |
R-free | 0.24790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5zx3 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.850 |
High resolution limit [Å] | 2.794 | 7.590 | 2.800 |
Rmerge | 0.111 | 0.041 | 1.020 |
Rmeas | 0.122 | 0.045 | 1.135 |
Rpim | 0.049 | 0.018 | 0.482 |
Number of reflections | 109729 | 5668 | 4652 |
<I/σ(I)> | 5.7 | ||
Completeness [%] | 97.5 | 98.6 | 83.1 |
Redundancy | 5.5 | 5.4 | 4.7 |
CC(1/2) | 0.998 | 0.608 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.08 M Magnesium acetate, 0.05 M sodium cacodylate pH 6.5, 15% PEG 400 |