6KON
Mycobacterium tuberculosis initial transcription complex comprising sigma H and 5'-OH RNA of 5 nt
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-23 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.97776 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 129.330, 161.219, 129.469 |
Unit cell angles | 90.00, 117.78, 90.00 |
Refinement procedure
Resolution | 48.651 - 3.000 |
R-factor | 0.2212 |
Rwork | 0.220 |
R-free | 0.27050 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5zx3 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.050 |
High resolution limit [Å] | 2.998 | 8.130 | 3.000 |
Rmerge | 0.105 | 0.040 | 1.072 |
Rmeas | 0.120 | 0.045 | 1.244 |
Rpim | 0.057 | 0.021 | 0.617 |
Number of reflections | 91565 | 4699 | 4076 |
<I/σ(I)> | 6.4 | ||
Completeness [%] | 97.8 | 97.9 | 88 |
Redundancy | 4.2 | 4.2 | 3.6 |
CC(1/2) | 0.997 | 0.506 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.08 M Magnesium acetate, 0.05 M sodium cacodylate pH 6.5, 10% PEG 400 |