6KOJ
Crystal structure of SNX11-PXe domain in complex with PI(3,5)P2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-09-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 |
| Unit cell lengths | 31.860, 45.270, 50.480 |
| Unit cell angles | 110.19, 106.04, 90.07 |
Refinement procedure
| Resolution | 45.270 - 2.140 |
| R-factor | 0.2049 |
| Rwork | 0.201 |
| R-free | 0.27090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ikb |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.717 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.270 | 45.270 | 2.210 |
| High resolution limit [Å] | 2.140 | 9.090 | 2.140 |
| Rmerge | 0.098 | 0.102 | 0.234 |
| Rmeas | 0.118 | 0.125 | 0.279 |
| Rpim | 0.064 | 0.071 | 0.150 |
| Total number of observations | 40277 | 498 | 3411 |
| Number of reflections | 12451 | 164 | 1039 |
| <I/σ(I)> | 8.1 | 12.1 | 4 |
| Completeness [%] | 89.6 | 89.1 | 91.5 |
| Redundancy | 3.2 | 3 | 3.3 |
| CC(1/2) | 0.981 | 0.954 | 0.934 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 293 | 0.1 M NaAc pH 4.6, 2% v/v Tacsimate pH 4.0, 16% w/v PEG 3350 |
