6KOI
Crystal structure of SNX11-PXe domain in dimer form.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 142.040, 190.434, 237.012 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.870 - 3.500 |
| R-factor | 0.2359 |
| Rwork | 0.234 |
| R-free | 0.26540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ikb |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.526 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 66.540 | 66.540 | 3.570 |
| High resolution limit [Å] | 3.500 | 18.190 | 3.500 |
| Rmerge | 0.131 | 0.073 | 0.819 |
| Rmeas | 0.143 | 0.080 | 0.892 |
| Rpim | 0.056 | 0.033 | 0.347 |
| Total number of observations | 507560 | 3339 | 27708 |
| Number of reflections | 80923 | 646 | 4350 |
| <I/σ(I)> | 11.3 | 22.2 | 2.6 |
| Completeness [%] | 99.2 | 96.7 | 98.6 |
| Redundancy | 6.3 | 5.2 | 6.4 |
| CC(1/2) | 0.995 | 0.989 | 0.699 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.3 | 293 | 0.1 M sodium acetate trihydrate pH 4.3, 1.8 M sodium formate |
