6KND
The C-terminal Domain of Translation Initiation Factor 5 at low pH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-01-18 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.1 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 48.494, 68.649, 126.815 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.608 - 1.900 |
R-factor | 0.2165 |
Rwork | 0.214 |
R-free | 0.25710 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.806 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 39.608 | 39.608 | 2.010 |
High resolution limit [Å] | 1.900 | 5.650 | 1.900 |
Rmerge | 0.045 | 0.016 | 0.652 |
Rmeas | 0.051 | 0.018 | 0.742 |
Total number of observations | 73362 | ||
Number of reflections | 17005 | 726 | 2581 |
<I/σ(I)> | 19.24 | 59.27 | 2.37 |
Completeness [%] | 98.8 | 99.2 | 94.1 |
Redundancy | 4.314 | 4.077 | 4.307 |
CC(1/2) | 0.999 | 1.000 | 0.901 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | sodium chloride, phosphate-citrate buffer (pH 4.2), 50% (v/v) PEG 200 |