6KMQ

2.3 Angstrom resolution structure of dimeric HigBA toxin-antitoxin complex from E. coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-27
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.918400
Spacegroup name	P 1 21 1
Unit cell lengths	37.960, 48.270, 56.070
Unit cell angles	90.00, 100.82, 90.00

Refinement procedure

Resolution	28.492 - 2.350
R-factor	0.2621
Rwork	0.260
R-free	0.29750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ifg
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.492	2.309
High resolution limit [Å]	2.300	2.300
Number of reflections	8815	1421
<I/σ(I)>	13.16
Completeness [%]	97.5
Redundancy	3.75
CC(1/2)	0.999	0.887

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.05M MgCl2, 0.2M KCl, 0.1M Tris pH 7.5, 20% PEG 3350