6KMD

Crystal structure of SeMet-phytochromobilin synthase from tomato in complex with biliverdin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-13
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.975
Spacegroup name	P 43 21 2
Unit cell lengths	63.925, 63.925, 158.426
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.476 - 2.200
Rwork	0.177
R-free	0.21150
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.14-3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.770	2.330
High resolution limit [Å]	2.200	2.200
Number of reflections	31770	5053
<I/σ(I)>	13.21	2.88
Completeness [%]	99.8	98.9
Redundancy	6.97	6.8
CC(1/2)	0.997	0.864

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	PEG 8000, magnesium chloride, Tris-HCl