6KLY
Crystal structure of the type III effector XopAI from Xanthomonas axonopodis pv. citri in space group P43212
Replaces: 4ELNExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13C1 |
Synchrotron site | NSRRC |
Beamline | BL13C1 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2010-06-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9762 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 73.050, 73.050, 114.060 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.563 - 2.010 |
R-factor | 0.1671 |
Rwork | 0.166 |
R-free | 0.19490 |
Structure solution method | MAD |
Data reduction software | iMOSFLM (v7.2.2) |
Data scaling software | pointless (v1.11.21) |
Phasing software | PHENIX (1.14_3260: ???) |
Refinement software | PHENIX (1.14_3260: ???) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.350 | 2.060 |
High resolution limit [Å] | 2.010 | 2.010 |
Rmerge | 0.082 | 0.525 |
Rmeas | 0.094 | 0.605 |
Rpim | 0.043 | 0.290 |
Number of reflections | 19639 | 1275 |
<I/σ(I)> | 11.1 | |
Completeness [%] | 93.7 | 85.3 |
Redundancy | 4.1 | 3.7 |
CC(1/2) | 0.997 | 0.712 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 15% (v/v) PEG 400, 5.5% (w/v) PEG 20000, 0.05 M Potassium phosphate, pH 8.0 |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 15% (v/v) PEG 400, 5.5% (w/v) PEG 20000, 0.05 M Potassium phosphate, pH 8.0 |