6KLR

Crystal structure of human WIPI3 in complex with the WIR-peptide from ATG2A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97855
Spacegroup name	P 21 21 2
Unit cell lengths	75.124, 121.063, 37.768
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.520 - 2.210
R-factor	0.2147
Rwork	0.213
R-free	0.25530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6iyy
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.093	0.423
Rmeas	0.102	0.451
Rpim	0.029	0.149
Number of reflections	17842	1650
<I/σ(I)>	23.1	3.2
Completeness [%]	99.3	96.2
Redundancy	11.6	7.9
CC(1/2)	0.989	0.925

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.1M Tirs-HCl, pH 8.5, 20% (v/v) Ethanol