6KLI

Crystal Structure of the Zea Mays laccase 3 complexed with sinapyl

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-15
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97853
Spacegroup name	P 61 2 2
Unit cell lengths	112.213, 112.213, 207.562
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.678 - 1.800
R-factor	0.1763
Rwork	0.175
R-free	0.20070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1aoz
RMSD bond length	0.006
RMSD bond angle	0.872
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.700	1.830
High resolution limit [Å]	1.800	1.800
Rmerge	0.080	0.500
Number of reflections	71621	3480
<I/σ(I)>	26.8	5
Completeness [%]	99.4
Redundancy	8.4
CC(1/2)	0.998	0.925

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	291	5% of PEG 3350, 0.2 M (NH4)2SO4 and 0.1 M Bis-tris,pH 5.5