6KLG

Crystal Structure of the Zea Mays laccase 3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17B1
Synchrotron site	SSRF
Beamline	BL17B1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-05-14
Detector	RAYONIX MX-300
Wavelength(s)	1.03317
Spacegroup name	P 61 2 2
Unit cell lengths	111.977, 111.977, 208.094
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.484 - 2.100
R-factor	0.1656
Rwork	0.164
R-free	0.20010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1aoz
RMSD bond length	0.008
RMSD bond angle	0.924
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.140
High resolution limit [Å]	2.100	2.100
Rmerge	0.145	0.508
Number of reflections	45720	2234
<I/σ(I)>	23	6.7
Completeness [%]	100.0
Redundancy	15.6
CC(1/2)	0.999	0.963

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	291	15% of PEG 3350, 0.2 M (NH4)2SO4 and 0.1 M Bis-tris,pH 5.5