6KL5

Structure of The N-terminal domain of Middle East respiratory syndrome coronavirus Nucleocapsid Protein complexed with Benzyl 2-(Hydroxymethyl)-1-Indolinecarboxylate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13C1
Synchrotron site	NSRRC
Beamline	BL13C1
Temperature [K]	110
Detector technology	CCD
Collection date	2015-09-26
Detector	RAYONIX MX300HE
Wavelength(s)	0.97622
Spacegroup name	P 1 21 1
Unit cell lengths	35.440, 109.897, 91.526
Unit cell angles	90.00, 101.10, 90.00

Refinement procedure

Resolution	26.289 - 3.090
R-factor	0.2829
Rwork	0.281
R-free	0.29880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4j3k
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	6.640	3.200
High resolution limit [Å]	3.076	6.640	3.090
Rmerge	0.137	0.042	0.481
Rmeas	0.161	0.050	0.567
Rpim	0.084	0.026	0.298
Number of reflections	12334	1310	1266
<I/σ(I)>	5.6
Completeness [%]	96.1	99.1	96.9
Redundancy	3.5	3.7	3.4
CC(1/2)		0.996	0.788

Crystallization Conditions

crystal ID	method	pH	temperature	details
1