6KL4

Crystal structure of MavC-UBE2N-Ub

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97894
Spacegroup name	P 65
Unit cell lengths	149.561, 149.561, 58.774
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.000 - 2.850
R-factor	0.1887
Rwork	0.185
R-free	0.26180
Structure solution method	SAD
Data scaling software	xia2
Phasing software	HKL-3000
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.000	2.900
High resolution limit [Å]	2.850	2.850
Rmerge	0.103	1.275
Number of reflections	17794	882
<I/σ(I)>	35.5	2.6
Completeness [%]	99.9
Redundancy	20
CC(1/2)	1.000	0.940

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	COUNTER-DIFFUSION		289.15	0.2M Sodium malonate pH 6.0, 20%w/v PEG 3,350