6KL2

Structure of the N-terminal domain of Middle East respiratory syndrome coronavirus nucleocapsid protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	110
Detector technology	CCD
Collection date	2015-04-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00000
Spacegroup name	P 1 21 1
Unit cell lengths	35.601, 109.645, 91.993
Unit cell angles	90.00, 101.23, 90.00

Refinement procedure

Resolution	23.472 - 2.630
R-factor	0.281
Rwork	0.280
R-free	0.28980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4j3k
RMSD bond length	0.013
RMSD bond angle	1.590
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.720
High resolution limit [Å]	2.627	5.650	2.630
Rmerge	0.095	0.037	0.493
Rmeas	0.103	0.040	0.540
Rpim	0.038	0.014	0.216
Number of reflections	19546	2066	1872
<I/σ(I)>	8.3
Completeness [%]	94.5	97.5	91.4
Redundancy	7.1	7.4	5.9
CC(1/2)		0.998	0.841

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	5 mg/mL protein, 25 mM Tris-HCl, pH 7.5, 75 mM NaCl, 70 mM MES, pH5.5, 37.5 M (NH4)2SO4, 14.5 % PEG 3350, 1 mM NaBr