6KL1
Crystal structure of the S65T/F99S/M153T/V163A variant of non-deuterated GFP at pD 8.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 50 |
| Detector technology | CCD |
| Collection date | 2017-07-21 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.75 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.915, 62.107, 68.865 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.457 - 0.851 |
| R-factor | 0.1041 |
| Rwork | 0.103 |
| R-free | 0.11790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wur |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 0.860 |
| High resolution limit [Å] | 0.850 | 0.850 |
| Rmerge | 0.082 | 1.258 |
| Number of reflections | 189497 | 9243 |
| <I/σ(I)> | 21.6 | 1.3 |
| Completeness [%] | 99.3 | 98 |
| Redundancy | 8.5 | 6.2 |
| CC(1/2) | 0.526 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.1 | 308 | PEG 4000, MgCl2, Tris-DCl buffer |
