6KKZ
Crystal structure of the S65T/F99S/M153T/V163A variant of perdeuterated GFP at pD 8.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-13 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.70 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.762, 62.112, 68.911 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.137 - 0.900 |
| R-factor | 0.108 |
| Rwork | 0.107 |
| R-free | 0.12350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wur |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.442 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 0.920 |
| High resolution limit [Å] | 0.900 | 0.900 |
| Rmerge | 0.094 | 0.839 |
| Number of reflections | 159331 | 6457 |
| <I/σ(I)> | 18 | 1 |
| Completeness [%] | 98.3 | 80.3 |
| Redundancy | 5.8 | 3 |
| CC(1/2) | 0.473 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.1 | 308 | PEG 4000, MgCl2, Tris-DCl buffer |
