6KKS
Structural insights into target DNA recognition by R2R3-type MYB transcription factor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-05-31 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97915 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.084, 63.927, 73.956 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.150 |
R-factor | 0.215 |
Rwork | 0.214 |
R-free | 0.24560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1h8a |
RMSD bond length | 0.006 |
RMSD bond angle | 1.149 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.364 | 48.364 | 2.280 |
High resolution limit [Å] | 2.150 | 6.400 | 2.150 |
Rmerge | 0.105 | 0.043 | 0.935 |
Rmeas | 0.109 | 0.045 | 0.969 |
Total number of observations | 198226 | ||
Number of reflections | 13918 | 590 | 2214 |
<I/σ(I)> | 21.04 | 55.3 | 3.5 |
Completeness [%] | 99.6 | 99.2 | 100 |
Redundancy | 14.242 | 12.627 | 14.374 |
CC(1/2) | 0.999 | 0.999 | 0.924 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1M MES pH 6.0, 30%(v/v) PEG600, 5%(w/v) PEG1000 and 10%(v/v) Glycerol |