6KJY

Galectin-13 variant C136S/C138S

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97
Spacegroup name	P 43 21 2
Unit cell lengths	53.510, 53.510, 113.766
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.499 - 1.500
R-factor	0.1869
Rwork	0.185
R-free	0.20500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.006
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.500	1.530
High resolution limit [Å]	1.500	1.500
Number of reflections	27251	1260
<I/σ(I)>	35.7
Completeness [%]	99.6
Redundancy	11.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	Bis-Tris