6KJL
Xylanase J from Bacillus sp. strain 41M-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-03 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.90000 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 125.940, 125.940, 318.570 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.000 - 2.450 |
R-factor | 0.1705 |
Rwork | 0.169 |
R-free | 0.19750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2dcj |
RMSD bond length | 0.008 |
RMSD bond angle | 1.032 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX (1.15_3459) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.395 | 2.600 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.064 | 0.632 |
Rmeas | 0.077 | 0.642 |
Number of reflections | 55529 | 16645 |
<I/σ(I)> | 13.3 | 2.06 |
Completeness [%] | 98.6 | 99.5 |
Redundancy | 2.59 | 3.09 |
CC(1/2) | 0.997 | 0.698 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | HEPES, sodium citrate, 2-methyl-2,4-pentanediol, ethylene glycol |