6KJI
Crystal structure of PsoF with SAH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-17 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.980000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 190.873, 44.036, 143.124 |
| Unit cell angles | 90.00, 93.24, 90.00 |
Refinement procedure
| Resolution | 19.700 - 1.990 |
| R-factor | 0.1739 |
| Rwork | 0.172 |
| R-free | 0.21440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6kjg |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.100 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.031 | 0.130 |
| Number of reflections | 82243 | 12967 |
| <I/σ(I)> | 24.9 | |
| Completeness [%] | 99.3 | 98.6 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG 3350, sodium fluoride, bis-tris propane pH 6.5 |
