6KJG
Crystal structure of PsoF
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-11-11 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.98000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 189.238, 44.102, 142.616 |
Unit cell angles | 90.00, 93.59, 90.00 |
Refinement procedure
Resolution | 19.800 - 1.990 |
R-factor | 0.1982 |
Rwork | 0.197 |
R-free | 0.22960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5thy |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.090 |
High resolution limit [Å] | 1.990 | 1.990 |
Rmerge | 0.054 | 0.332 |
Number of reflections | 80787 | 12540 |
<I/σ(I)> | 11.87 | 2.48 |
Completeness [%] | 98.3 | 95.8 |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG 3350, sodium fluoride, bis-tris propane pH 6.5 |