6KJG

Crystal structure of PsoF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-11
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.98000
Spacegroup name	C 1 2 1
Unit cell lengths	189.238, 44.102, 142.616
Unit cell angles	90.00, 93.59, 90.00

Refinement procedure

Resolution	19.800 - 1.990
R-factor	0.1982
Rwork	0.197
R-free	0.22960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5thy
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.090
High resolution limit [Å]	1.990	1.990
Rmerge	0.054	0.332
Number of reflections	80787	12540
<I/σ(I)>	11.87	2.48
Completeness [%]	98.3	95.8
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 3350, sodium fluoride, bis-tris propane pH 6.5