6KIQ
Complex of yeast cytoplasmic dynein MTBD-High and MT with DTT
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TALOS ARCTICA |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.7 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 3.62 |
| Number of Particle | 35636 |
| Software | FREALIGN,Gctf,MDFF,RELION |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
