6KID

Crystal structure of human leucyl-tRNA synthetase, ATP-bound form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	273
Detector technology	PIXEL
Collection date	2019-04-12
Detector	DECTRIS EIGER2 X 4M
Wavelength(s)	1.0000
Spacegroup name	P 65 2 2
Unit cell lengths	137.224, 137.224, 433.321
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.890 - 3.150
R-factor	0.2473
Rwork	0.245
R-free	0.29260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wz2
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.010
High resolution limit [Å]	2.960	8.020	2.960
Rmerge	0.170	0.051	0.409
Rmeas	0.202	0.056	0.543
Rpim	0.106	0.022	0.352
Total number of observations	230992
Number of reflections	82644	4636	3251
<I/σ(I)>	5
Completeness [%]	88.5	98.4	70.6
Redundancy	2.8	5.6	1.6
CC(1/2)		0.999	0.001

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M HEPES (pH 7.1), 0.42 M Ammonium sulfate, 24 % PEG3350