6KI2
The STAS domain of cyanobacteria bicarbonate transporter BicA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-02 |
Detector | DECTRIS PILATUS3 R CdTe 300K |
Wavelength(s) | 0.9793 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.050, 62.109, 57.036 |
Unit cell angles | 90.00, 119.86, 90.00 |
Refinement procedure
Resolution | 28.581 - 2.197 |
Rwork | 0.213 |
R-free | 0.23010 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.013 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.280 |
High resolution limit [Å] | 2.197 | 4.730 | 2.200 |
Rmerge | 0.103 | 0.083 | 0.354 |
Rmeas | 0.113 | 0.091 | 0.389 |
Rpim | 0.046 | 0.038 | 0.159 |
Number of reflections | 34281 | 1822 | 1757 |
<I/σ(I)> | 6.2 | ||
Completeness [%] | 99.8 | 99.7 | 99.9 |
Redundancy | 6.1 | 6.1 | 5.8 |
CC(1/2) | 0.978 | 0.938 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20 mM Magnesium chloride, 2 mM Cobalt chloride, 50 mM HEPES, pH7.5, 2.0 M Ammonium sulphate, 1 mM spermine. |