6KHZ
p62/SQSTM1 ZZ domain with Gly-peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 173 |
Detector technology | IMAGE PLATE |
Collection date | 2017-04-14 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.0000 |
Spacegroup name | I 2 3 |
Unit cell lengths | 113.979, 113.979, 113.979 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.300 - 2.800 |
R-factor | 0.24 |
Rwork | 0.235 |
R-free | 0.28730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5yp7 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.850 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.130 | 0.860 |
Rpim | 0.054 | |
Number of reflections | 6200 | 6200 |
<I/σ(I)> | 19.8 | |
Completeness [%] | 99.9 | |
Redundancy | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.17 M Ammonium sulfate, 0.085 M Sodium cacodylate trihydrate pH 6.5, 22-30 % w/v Polyethylene glycol 8000 |