6KHU
The crystal structure of a DGC protein from Thermotoga maritima
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-16 |
Detector | BRUKER SMART 6500 |
Wavelength(s) | 0.9789 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 61.303, 61.303, 101.143 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.542 - 2.098 |
R-factor | 0.2001 |
Rwork | 0.196 |
R-free | 0.23760 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 0.847 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.140 |
High resolution limit [Å] | 2.098 | 5.690 | 2.100 |
Rmerge | 0.102 | 0.030 | 0.454 |
Number of reflections | 21596 | 1075 | 1087 |
<I/σ(I)> | 10.6 | ||
Completeness [%] | 99.9 | 98.4 | 99.9 |
Redundancy | 14.5 | 14.3 | 14.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 295 | PEG 3000, trisodium citrate, Zinc acetate |