6KHR
Structure of glycinamide-RNase-transformylase T from Mycobacterium tuberculosis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-18 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.978 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 103.132, 103.132, 148.692 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.719 - 2.786 |
R-factor | 0.2179 |
Rwork | 0.217 |
R-free | 0.24220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1eyz |
Data reduction software | MOSFLM |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.14rc3_3206) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.719 | 2.940 |
High resolution limit [Å] | 2.786 | 2.790 |
Rmerge | 0.266 | 0.874 |
Rpim | 0.098 | 0.320 |
Number of reflections | 23300 | 27385 |
<I/σ(I)> | 8 | 4.7 |
Completeness [%] | 99.4 | 97 |
Redundancy | 8.9 | 8.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.06 M sodium acetate, pH4.6, 6.0% PEG4000, 32% glycerol. |