6KHO

Crystal structure of Oryza sativa TDC with PLP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-30
Detector	DECTRIS PILATUS3 R CdTe 300K
Wavelength(s)	0.97915
Spacegroup name	P 31 2 1
Unit cell lengths	155.733, 155.733, 102.182
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.717 - 1.972
R-factor	0.1699
Rwork	0.169
R-free	0.19450
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.030
High resolution limit [Å]	1.970	1.970
Rmerge	0.083	0.625
Number of reflections	98063	9359
<I/σ(I)>	39.6	2.476
Completeness [%]	97.5	93.6
Redundancy	9.3	9.3
CC(1/2)		0.844

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	PEG 1000, Imidazole/Hydrochloric acid, Calcium acetate