6KFU

A ACP-AMT Fusion Protein of Hybrid Polyketide/Non-Ribosomal Peptide Synthetase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9791
Spacegroup name	P 31 1 2
Unit cell lengths	73.939, 73.939, 209.617
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.410 - 2.200
Rwork	0.249
R-free	0.29700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6kfm
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.13-2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.033	2.135
High resolution limit [Å]	2.034	2.034
Rmerge	0.109	0.931
Rmeas	0.113	0.972
Rpim	0.026	0.275
Number of reflections	36874	1845
<I/σ(I)>	15.121	1.448
Completeness [%]	89.5	32.1
Redundancy	18.6
CC(1/2)	0.998	0.665

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.7	312	0.1 M sodium citrate, pH 5.7, 18% (v/v) PEG 20000