6KFS

Structure of CCM related protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	H 3 2
Unit cell lengths	93.363, 93.363, 177.834
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.710 - 1.200
Rwork	0.149
R-free	0.16680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.976
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.230
High resolution limit [Å]	1.200	1.200
Number of reflections	93030	6859
<I/σ(I)>	17.96
Completeness [%]	100.0
Redundancy	22

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2M (NH4)SO4, 0.1M Bis-Tris pH 5.5, 25% PEG 3350