6KFP
Crystal structure of MavC ternary complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-18 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.979 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 97.704, 105.860, 125.391 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.530 - 2.920 |
R-factor | 0.196 |
Rwork | 0.193 |
R-free | 0.24980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5tsc 1ubq |
RMSD bond length | 0.003 |
RMSD bond angle | 0.606 |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER (1.15_3459) |
Refinement software | PHENIX (1.15_3459) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.020 |
High resolution limit [Å] | 2.920 | 2.920 |
Rmerge | 0.098 | 0.794 |
Number of reflections | 14312 | 1390 |
<I/σ(I)> | 26.6 | |
Completeness [%] | 99.4 | |
Redundancy | 12 | |
CC(1/2) | 0.997 | 0.882 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG 3350, lithium chloride |