6KFK
Structure of Salmonella flagellar hook reveals intermolecular domain interactions for the universal joint function
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | JEOL 3200FSC |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 4.1 |
| Energy Filter | In-column Omega Filter |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 4.1 |
| Number of Particle | 42293 |
| Software | RELION,RosettaEM,PHENIX,Gctf,UCSF Chimera,Coot |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
