6KEV

Reduced phosphoribulokinase from Synechococcus elongatus PCC 7942 complexed with adenosine diphosphate and glucose 6-phosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.98
Spacegroup name	P 2 21 21
Unit cell lengths	46.415, 77.104, 94.405
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.135 - 2.506
R-factor	0.191333603136
Rwork	0.190
R-free	0.22295
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.732
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.140	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	12171	1187
<I/σ(I)>	18.4
Completeness [%]	100.0
Redundancy	12.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.02 M magnesium chloride hexahydrate, 0.05 M MES monohydrate pH6.0, 15% v/v 2-propanol, 10mM adenosine diphosphate, 10mM glucose 6-phosphate