6KEM

Crystal structure of Drosophila melanogaster Noppera-bo, glutathione S-transferase epsilon 14 (DmGSTE14), in apo-form 2

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-23
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	59.120, 76.556, 106.358
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.830 - 1.500
R-factor	0.1658
Rwork	0.164
R-free	0.19470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6kel
RMSD bond length	0.015
RMSD bond angle	1.316
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.16_3549, 1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.830	1.554
High resolution limit [Å]	1.500	1.500
Rmerge	0.045	0.383
Rmeas	0.046	0.399
Rpim		0.111
Number of reflections	77842	7648
<I/σ(I)>	33.77	6.53
Completeness [%]	100.0	99.42
Redundancy	13.1	12.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	293	42.5% (v/v) PPG400, 100 mM Bis-Tris, and pH 6.4